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Anti-Human USP4 Polyclonal Antibody
Anti-Human USP4 Polyclonal Antibody
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Спецификация:
  • 20ug
  • 50ug
  • 100ug
Количество:
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Обзор
Каталожный номерHC280014
Описание
Anti-Human USP4 Polyclonal Antibody (HC280014) is a rabbit polyclonal antibody detecting USP4 in ELISA, IHC, WB. Suitable for Human, Bovine, Rat, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human USP4 (Asp12-Glu220).
Цель Ubiquitin carboxyl-terminal hydrolase 4, 3.4.19.12, Deubiquitinating enzyme 4, Ubiquitin thioesterase 4, Ubiquitin-specific-processing protease 4, Ubiquitous nuclear protein homolog, USP4, UNP, UNPH
Уровень эндотоксинов Please contact with the lab for this information.
Наблюдаемая молекулярная масса ~110 kDa
Очистка Purified by antigen affinity column.
Номер доступа Q13107, A6QR55, B2GUZ1, P35123
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Ubiquitin carboxyl-terminal hydrolase 4 (USP4) is a ~108 kDa protein. Deubiquitinating enzyme that removes conjugated ubiquitin from target proteins. Deubiquitinates PDPK1. Deubiquitinates TRIM21. Deubiquitinates receptor ADORA2A which increases the amount of functional receptor at the cell surface. Deubiquitinates HAS2.

1. Wada, K. et al. (2006) Biochemical and biophysical research communications 339, 731-6. PMID: 16316627
2. Milojevic, T. et al. (2006) Molecular pharmacology 69, 1083-94. PMID: 16339847
3. Wada, K. et al. (2006) Biochemical and biophysical research communications 342, 253-8. PMID: 16472766
4. Song, EJ. et al. (2010) Genes & development 24, 1434-47. PMID: 20595234
5. Uras, IZ. et al. (2012) PloS one 7, e31003. PMID: 22347420
6. Clerici, M. et al. (2014) Nature communications 5, 5399. PMID: 25404403
7. Mehić, M. et al. (2017) Oncogenesis 6, e348. PMID: 28604766
8. Deng, L. et al. (2019) Cell research 29, 136-150. PMID: 30514904
9. Chu, F. et al. (2024) Proceedings of the National Academy of Sciences of the United States of America 121, e2401174121. PMID: 39589880
Примечание For research use only.
Изображения
  • Anti-Human USP4 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-USP4 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: Jurkat cell lysate

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
Рекомендация

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