









| Numéro de catalogue | HB941013 | ||||
|---|---|---|---|---|---|
| Reactivité des espèces | Human | ||||
| Applications | ELISA, FCM | ||||
| Espèce hôte | Rabbit | ||||
| Clonalité | Monoclonal | ||||
| Isotype | IgG | ||||
| ID de clone | SAA0531 | ||||
| Applications testées | FCM | ||||
| Cible | L-VEGF, N-VEGF, VEGF, VEGFA, VPF, Vascular endothelial growth factor A, long form, Vascular permeability factor | ||||
| Niveau d'endotoxines | < 10 EU/mg | ||||
| Pureté | >95% as determined by SDS-PAGE. | ||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||
| Numéro d'accès | P15692 | ||||
| Forme | Liquid | ||||
| Tampon de stockage | 0.01M PBS pH 7.4. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot. |
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| Informations d'utilisation du produit |
| ||||
| Stabilité et stockage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||
| Note | For research use only. | ||||
| Isotype Control | Rabbit IgG Recombinant Isotype Control Antibody (HyHEL-10) (TF690107) |

Detects Human VEGFA in indirect ELISAs.

Flow-cytometry using anti-human VEGFA antibody.VEGFA Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VEGFA antibody monoclonal antibody (Catalog # HB941013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody (Catalog # TF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human VEGFA antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VEGFA antibody monoclonal antibody (Catalog # HB941013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody (Catalog # TF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human VEGFA antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VEGFA antibody monoclonal antibody (Catalog # HB941013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody (Catalog # TF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human VEGFA Antibody (SAA0531).




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