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| カタログ番号 | HB941013 | ||||
|---|---|---|---|---|---|
| 種反応性 | Human | ||||
| アプリケーション | ELISA, FCM | ||||
| 宿主種 | Rabbit | ||||
| クローン性 | Monoclonal | ||||
| アイソタイプ | IgG | ||||
| クローンID | SAA0531 | ||||
| テスト済みアプリケーション | FCM | ||||
| ターゲット | L-VEGF, N-VEGF, VEGF, VEGFA, VPF, Vascular endothelial growth factor A, long form, Vascular permeability factor | ||||
| エンドトキシンレベル | < 10 EU/mg | ||||
| 純度 | >95% as determined by SDS-PAGE. | ||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||
| アクセッション番号 | P15692 | ||||
| 形態 | Liquid | ||||
| 保存バッファー | 0.01M PBS pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||
| 注意事項 | For research use only. | ||||
| Isotype Control | Rabbit IgG Recombinant Isotype Control Antibody (HyHEL-10) (TF690107) |
Detects Human VEGFA in indirect ELISAs.
Flow-cytometry using anti-human VEGFA antibody.VEGFA Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VEGFA antibody monoclonal antibody (Catalog # HB941013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody (Catalog # TF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human VEGFA antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VEGFA antibody monoclonal antibody (Catalog # HB941013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody (Catalog # TF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human VEGFA antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VEGFA antibody monoclonal antibody (Catalog # HB941013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody (Catalog # TF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for Anti-Human VEGFA Antibody (SAA0531).
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