Anti-Human Vimentin Recombinant Antibody (VB6) (HY550107)

Name: Anti-Human Vimentin Recombinant Antibody (VB6)
Brand: abinScience
SKU: HY550107
Price: 191 USD
Availability: InStock

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Vista general

Número de catálogo	HY550107
Reactividad de especies	Human
Aplicaciones	ELISA, FCM, WB
Especie huésped	Human
Clonalidad	Monoclonal
Isotipo	IgG1, kappa
ID de clon	VB6
Aplicaciones probadas	FCM
Objetivo	Vimentin, VIM, RHC35301
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>95% as determined by SDS-PAGE.
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	P08670
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4 Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo	Vimentin is a ~53 kDa protein. Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front. Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner. May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips.
Nota	For research use only.

Imágenes

Bioactivity

SEC-HPLC detection for Anti-Human VIM/Vimentin Antibody (VB6).
Flow Cytometry

Flow-cytometry using anti-human VIM antibody.VIM/Vimentin Transfected HI5 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VIM antibody monoclonal antibody (Catalog # HY550107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human VIM antibody.PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VIM antibody monoclonal antibody (Catalog # HY550107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.