Anti-Human Vimentin Recombinant Antibody (VB6) (HY550107)

Name: Anti-Human Vimentin Recombinant Antibody (VB6)
Brand: abinScience
SKU: HY550107
Price: 191 USD
Availability: InStock

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概要
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参考文献
データシート
SDS

概要

カタログ番号	HY550107
種反応性	Human
アプリケーション	ELISA, FCM, WB
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG1, kappa
クローンID	VB6
テスト済みアプリケーション	FCM
ターゲット	Vimentin, VIM, RHC35301
エンドトキシンレベル	Please contact with the lab for this information.
純度	>95% as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P08670
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Vimentin is a ~53 kDa protein. Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front. Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner. May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips.
注意事項	For research use only.

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Bioactivity

SEC-HPLC detection for Anti-Human VIM/Vimentin Antibody (VB6).
Flow Cytometry

Flow-cytometry using anti-human VIM antibody.VIM/Vimentin Transfected HI5 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VIM antibody monoclonal antibody (Catalog # HY550107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human VIM antibody.PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VIM antibody monoclonal antibody (Catalog # HY550107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.