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| カタログ番号 | HP969014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human VPS16 (Lys492-Lys839). | ||||||||
| ターゲット | hVPS16, VPS16, Vacuolar protein sorting-associated protein 16 homolog | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q9H269 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Vacuolar protein sorting-associated protein 16 homolog (VPS16) is a ~94 kDa protein. Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for recruitment of VPS33A to the HOPS complex. 1. Kim, BY. et al. (2001) The Journal of biological chemistry 276, 29393-402. PMID: 11382755 2. Solinger, JA. et al. (2013) The FEBS journal 280, 2743-57. PMID: 23351085 3. Jiang, P. et al. (2014) Molecular biology of the cell 25, 1327-37. PMID: 24554770 4. Perini, ED. et al. (2014) Traffic (Copenhagen, Denmark) 15, 1366-89. PMID: 25266290 5. Wartosch, L. et al. (2015) Traffic (Copenhagen, Denmark) 16, 727-42. PMID: 25783203 6. Graham, SC. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 13345-50. PMID: 23901104 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-VPS16 polyclonal antibody at 1μg/ml on various samples.
Lane 1: A549 cell lysate
Lane 2: MOLT-4 cell lysate
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