Anti-Human WDR4 Polyclonal Antibody (HD097014)

Name: Anti-Human WDR4 Polyclonal Antibody
Brand: abinScience
SKU: HD097014
Price: 168 USD
Availability: InStock

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Precio(USD): --

Especificación:

50ug
100ug

Número:

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Imágenes
Referencias
Datasheet
SDS

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Número de catálogo

HD097014

Descripción

Anti-Human WDR4 Polyclonal Antibody (HD097014) is a rabbit polyclonal antibody detecting WDR4. Suitable for Human.

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human WDR4 (Met1-Cys412).

Objetivo

Protein Wuho homolog, WD repeat-containing protein 4, WDR4, hWH, tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4

Nivel de endotoxinas

Please contact with the lab for this information.

Purificación

Purified by antigen affinity column.

Número de acceso

P57081

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Fondo

tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4 is a ~45 kDa protein. Non-catalytic component of the METTL1-WDR4 methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs). In the METTL1-WDR4 methyltransferase complex, WDR4 acts as a scaffold for tRNA-binding. Required for the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'-RAGGU-3' motif within the variable loop. M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay. Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs.

1. Alexandrov, A. et al. (2002) RNA (New York, N.Y.) 8, 1253-66. PMID: 12403464

2. Pandolfini, L. et al. (2019) Molecular cell 74, 1278-1290.e9. PMID: 31031083

3. Zhang, LS. et al. (2019) Molecular cell 74, 1304-1316.e8. PMID: 31031084

4. Ruiz-Arroyo, VM. et al. (2023) Nature 613, 383-390. PMID: 36599982

5. Li, J. et al. (2023) Nature 613, 391-397. PMID: 36599985

6. Jin, X. et al. (2023) Cell discovery 9, 65. PMID: 37369656

7. Dai, Z. et al. (2021) Molecular cell 81, 3339-3355.e8. PMID: 34352206

8. Orellana, EA. et al. (2021) Molecular cell 81, 3323-3338.e14. PMID: 34352207

9. Zhao, Z. et al. (2023) Cell 186, 3208-3226.e27. PMID: 37379838

Nota

For research use only

Imágenes

Western blot

Western blot analysis was performed using anti-WDR4 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse kidney lysate
Lane 2: Rat kidney lysate

Referencias

Datasheet

Anti-Human WDR4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-Human WDR4 Polyclonal Antibody (HD097014)

Vista general

Imágenes

Referencias

Datasheet

SDS