Anti-Human WDR4 Polyclonal Antibody (HD097014)

Name: Anti-Human WDR4 Polyclonal Antibody
Brand: abinScience
SKU: HD097014
Price: 168 USD
Availability: InStock

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Prix(USD) : --

Spécification :

50ug
100ug

Nombre :

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HD097014

Description

Anti-Human WDR4 Polyclonal Antibody (HD097014) is a rabbit polyclonal antibody detecting WDR4. Suitable for Human.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human WDR4 (Met1-Cys412).

Cible

Protein Wuho homolog, WD repeat-containing protein 4, WDR4, hWH, tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

P57081

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Contexte

tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4 is a ~45 kDa protein. Non-catalytic component of the METTL1-WDR4 methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs). In the METTL1-WDR4 methyltransferase complex, WDR4 acts as a scaffold for tRNA-binding. Required for the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'-RAGGU-3' motif within the variable loop. M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay. Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs.

1. Alexandrov, A. et al. (2002) RNA (New York, N.Y.) 8, 1253-66. PMID: 12403464

2. Pandolfini, L. et al. (2019) Molecular cell 74, 1278-1290.e9. PMID: 31031083

3. Zhang, LS. et al. (2019) Molecular cell 74, 1304-1316.e8. PMID: 31031084

4. Ruiz-Arroyo, VM. et al. (2023) Nature 613, 383-390. PMID: 36599982

5. Li, J. et al. (2023) Nature 613, 391-397. PMID: 36599985

6. Jin, X. et al. (2023) Cell discovery 9, 65. PMID: 37369656

7. Dai, Z. et al. (2021) Molecular cell 81, 3339-3355.e8. PMID: 34352206

8. Orellana, EA. et al. (2021) Molecular cell 81, 3323-3338.e14. PMID: 34352207

9. Zhao, Z. et al. (2023) Cell 186, 3208-3226.e27. PMID: 37379838

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-WDR4 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse kidney lysate
Lane 2: Rat kidney lysate

Reférences

Fiche technique

Anti-Human WDR4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-Human WDR4 Polyclonal Antibody (HD097014)

Aperçu

Images

Reférences

Fiche technique

SDS