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Anti-Human WDR4 Polyclonal Antibody
Anti-Human WDR4 Polyclonal Antibody
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Спецификация:
  • 50ug
  • 100ug
Количество:
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Обзор
Каталожный номерHD097014
Описание
Anti-Human WDR4 Polyclonal Antibody (HD097014) is a rabbit polyclonal antibody detecting WDR4. Suitable for Human.
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human WDR4 (Met1-Cys412).
Цель Protein Wuho homolog, WD repeat-containing protein 4, WDR4, hWH, tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4
Уровень эндотоксинов Please contact with the lab for this information.
Очистка Purified by antigen affinity column.
Номер доступа P57081
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Фон

tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4 is a ~45 kDa protein. Non-catalytic component of the METTL1-WDR4 methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs). In the METTL1-WDR4 methyltransferase complex, WDR4 acts as a scaffold for tRNA-binding. Required for the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'-RAGGU-3' motif within the variable loop. M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay. Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs.

1. Alexandrov, A. et al. (2002) RNA (New York, N.Y.) 8, 1253-66. PMID: 12403464
2. Pandolfini, L. et al. (2019) Molecular cell 74, 1278-1290.e9. PMID: 31031083
3. Zhang, LS. et al. (2019) Molecular cell 74, 1304-1316.e8. PMID: 31031084
4. Ruiz-Arroyo, VM. et al. (2023) Nature 613, 383-390. PMID: 36599982
5. Li, J. et al. (2023) Nature 613, 391-397. PMID: 36599985
6. Jin, X. et al. (2023) Cell discovery 9, 65. PMID: 37369656
7. Dai, Z. et al. (2021) Molecular cell 81, 3339-3355.e8. PMID: 34352206
8. Orellana, EA. et al. (2021) Molecular cell 81, 3323-3338.e14. PMID: 34352207
9. Zhao, Z. et al. (2023) Cell 186, 3208-3226.e27. PMID: 37379838
Примечание For research use only
Изображения
  • Anti-Human WDR4 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-WDR4 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse kidney lysate
    Lane 2: Rat kidney lysate

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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C₁ (Stock Conc.)
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Working Solution
C₂ (Working Conc.)
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