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Anti-Human WDR45 Polyclonal Antibody (HP297014)

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Aperçu
Numéro de catalogueHP297014
Description
Anti-Human WDR45 Polyclonal Antibody (HP297014) is a rabbit polyclonal antibody detecting WDR45 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human WDR45 (Cys23-Ala184).
Cible WD repeat-containing protein 45, WD repeat domain phosphoinositide-interacting protein 4, WDR45, WDRX1, WDRXI4, WIPI-4, WIPI4
Purification Purified by antigen affinity column.
Numéro d'accès Q9Y484
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

WD repeat domain phosphoinositide-interacting protein 4 (WDR45) is a ~39 kDa protein. Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Binds phosphatidylinositol 3-phosphate (PtdIns3P). Activated by the STK11/AMPK signaling pathway upon starvation, WDR45 is involved in autophagosome assembly downstream of WIPI2, regulating the size of forming autophagosomes. Together with WIPI1, promotes ATG2 (ATG2A or ATG2B)-mediated lipid transfer by enhancing ATG2-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes. Probably recruited to membranes through its PtdIns3P activity.

1. Saitsu, H. et al. (2013) Nature genetics 45, 445-9, 449e1. PMID: 23435086
2. Bakula, D. et al. (2017) Nature communications 8, 15637. PMID: 28561066
3. Maeda, S. et al. (2019) eLife 8. PMID: 31271352
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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C₂ (Working Conc.)
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Anti-Human WDR45 Polyclonal Antibody

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