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Anti-Human WNT1 Polyclonal Antibody (HY026014)

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Vista general
Número de catálogoHY026014
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human WNT1 (Gly32-Arg255).
Objetivo WNT1, Proto-oncogene Wnt-1, INT1, Proto-oncogene Int-1 homolog
Purificación Purified by antigen affinity column.
Número de acceso P04628
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Proto-oncogene Wnt-1 (WNT1) is a ~40 kDa protein. Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Plays an essential role in the development of the embryonic brain and central nervous system (CNS). Has a role in osteoblast function, bone development and bone homeostasis.

1. Keupp, K. et al. (2013) American journal of human genetics 92, 565-74. PMID: 23499309
2. Laine, CM. et al. (2013) The New England journal of medicine 368, 1809-16. PMID: 23656646
3. Mihara, E. et al. (2016) eLife 5. PMID: 26902720
4. Won, JY. et al. (2017) European journal of medical genetics 60, 411-415. PMID: 28528193
Nota For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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