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| カタログ番号 | HY120014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human XRCC6 Polyclonal Antibody (HY120014) is a rabbit polyclonal antibody detecting XRCC6 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human XRCC6 (Asp36-Lys238). | ||||||||
| ターゲット | Lupus Ku autoantigen protein p70, 5'-dRP lyase Ku70, Thyroid-lupus autoantigen, XRCC6, CTC box-binding factor 75 kDa subunit, X-ray repair complementing defective repair in Chinese hamster cells 6, G22P1, X-ray repair cross-complementing protein 6, ATP-dependent DNA helicase 2 subunit 1, DNA repair protein XRCC6, TLAA, ATP-dependent DNA helicase II 70 kDa subunit, CTCBF, 70 kDa subunit of Ku antigen, Ku70, 5'-deoxyribose-5-phosphate lyase Ku70, CTC75 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P12956 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | DNA repair protein Ku70 (XRCC6) is a ~69 kDa protein. DNA-binding protein critical for the DNA damage response, specifically in repairing double-strand breaks (DSBs) via the classical non-homologous end joining (NHEJ) pathway. It forms a heterodimer with XRCC5 (Ku80), creating the Ku70:Ku80 heterodimer (Ku complex), which serves as a DNA end-binding complex. It primarily binds DSBs and recruits essential repair factors, assembling the core long-range NHEJ complex to facilitate the alignment and ligation of broken DNA ends. This pathway ensures the rapid repair of cytotoxic and mutagenic DSBs and contributes to the generation of diversity in T-cell receptors and antibodies through mechanisms such as V(D)J recombination. Likely acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), catalyzing the beta-elimination of the 5'-deoxyribose-5-phosphate at abasic sites near DSBs. 1. Walker, JR. et al. (2001) Nature 412, 607-14. PMID: 11493912 2. Liu, H. et al. (2010) Biochemical and biophysical research communications 397, 245-50. PMID: 20493174 3. Chen, S. et al. (2021) Nature 593, 294-298. PMID: 33854234 4. Chaplin, AK. et al. (2021) Molecular cell 81, 3400-3409.e3. PMID: 34352203 5. West, RB. et al. (1998) Molecular and cellular biology 18, 5908-20. PMID: 9742108 6. Roberts, SA. et al. (2010) Nature 464, 1214-7. PMID: 20383123 7. Tuteja, N. et al. (1994) The EMBO journal 13, 4991-5001. PMID: 7957065 8. Maekawa, T. et al. (2018) Nucleic acids research 46, 4487-4504. PMID: 29490055 9. Willis, DM. et al. (2002) The Journal of biological chemistry 277, 37280-91. PMID: 12145306 10. Chung, U. et al. (1996) The Journal of biological chemistry 271, 8593-8. PMID: 8621488 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-XRCC6 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: Lncap cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: K562 cell lysate
Lane 6: 293T cell lysate
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