Anti-Human YWHAE Polyclonal Antibody (HX044014)

Name: Anti-Human YWHAE Polyclonal Antibody
Brand: abinScience
SKU: HX044014
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HX044014

説明

Anti-Human YWHAE Polyclonal Antibody (HX044014) is a rabbit polyclonal antibody detecting YWHAE in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human YWHAE (Met1-Gln255).

ターゲット

14-3-3E, YWHAE, 14-3-3 protein epsilon

精製

Purified by antigen affinity column.

アクセッション番号

P62258

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

14-3-3 protein epsilon (YWHAE) is a ~29 kDa protein. Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm. Plays a positive role in the antiviral signaling pathway upstream of TBK1 via interaction with RIGI.

1. Okamoto, Y. et al. (2011) The Journal of biological chemistry 286, 7171-81. PMID: 21189250

2. Zhang, ZD. et al. (2022) Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9, e2105391. PMID: 35343654

3. Wang, X. et al. (2003) Molecular and cellular biology 23, 6013-26. PMID: 12917326

4. Andrews, DDT. et al. (2023) Journal of virology 97, e0060423. PMID: 37555661

5. Liu, HM. et al. (2012) Cell host & microbe 11, 528-37. PMID: 22607805

6. Guo, M. et al. (2023) Cancer biology & therapy 24, 2246203. PMID: 37599448

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-YWHAE polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: A375 cell lysate
Lane 7: 293T cell lysate

参考文献

データシート

Anti-Human YWHAE Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-Human YWHAE Polyclonal Antibody (HX044014)

概要

イメージ

参考文献

データシート

SDS

クイックアクセス

お問い合わせ