Anti-Human YWHAG Polyclonal Antibody (HX994014)

Name: Anti-Human YWHAG Polyclonal Antibody
Brand: abinScience
SKU: HX994014
Price: 96 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HX994014

Description

Anti-Human YWHAG Polyclonal Antibody (HX994014) is a rabbit polyclonal antibody detecting YWHAG in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human YWHAG (Met1-Asn247).

Cible

Protein kinase C inhibitor protein 1, 14-3-3 protein gamma, YWHAG, KCIP-1

Purification

Purified by antigen affinity column.

Numéro d'accès

P61981

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

14-3-3 protein gamma (YWHAG) is a ~28 kDa protein. Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes inactivation of WDR24 component of the GATOR2 complex by binding to phosphorylated WDR24. Participates in the positive regulation of NMDA glutamate receptor activity by promoting the L-glutamate secretion through interaction with BEST1.

1. Yoshida, K. et al. (2005) Nature cell biology 7, 278-85. PMID: 15696159

2. Jin, Y. et al. (2006) The EMBO journal 25, 1207-18. PMID: 16511572

3. Dai, X. et al. (2023) Nature metabolism 5, 265-276. PMID: 36732624

4. Oh, SJ. et al. (2017) Molecular brain 10, 51. PMID: 29121962

5. Rietscher, K. et al. (2018) Journal of cell science 131. PMID: 29678907

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-YWHAG polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: A431 cell lysate
Lane 3: K562 cell lysate
Lane 4: A549 cell lysate
Lane 5: 293T cell lysate
Lane 6: Jurkat cell lysate
Lane 7: U251 cell lysate
Lane 8: Mouse lung lysate
Lane 9: Rat brain lysate
Lane 10: Rat testis lysate
Lane11: Rat lung lysate

Reférences

Fiche technique

Anti-Human YWHAG Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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