Anti-Human YWHAG Polyclonal Antibody (HX994014)

Name: Anti-Human YWHAG Polyclonal Antibody
Brand: abinScience
SKU: HX994014
Price: 96 USD
Availability: InStock

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Обзор

Каталожный номер

HX994014

Описание

Anti-Human YWHAG Polyclonal Antibody (HX994014) is a rabbit polyclonal antibody detecting YWHAG in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human YWHAG (Met1-Asn247).

Цель

Protein kinase C inhibitor protein 1, 14-3-3 protein gamma, YWHAG, KCIP-1

Очистка

Purified by antigen affinity column.

Номер доступа

P61981

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

14-3-3 protein gamma (YWHAG) is a ~28 kDa protein. Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes inactivation of WDR24 component of the GATOR2 complex by binding to phosphorylated WDR24. Participates in the positive regulation of NMDA glutamate receptor activity by promoting the L-glutamate secretion through interaction with BEST1.

1. Yoshida, K. et al. (2005) Nature cell biology 7, 278-85. PMID: 15696159

2. Jin, Y. et al. (2006) The EMBO journal 25, 1207-18. PMID: 16511572

3. Dai, X. et al. (2023) Nature metabolism 5, 265-276. PMID: 36732624

4. Oh, SJ. et al. (2017) Molecular brain 10, 51. PMID: 29121962

5. Rietscher, K. et al. (2018) Journal of cell science 131. PMID: 29678907

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-YWHAG polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: A431 cell lysate
Lane 3: K562 cell lysate
Lane 4: A549 cell lysate
Lane 5: 293T cell lysate
Lane 6: Jurkat cell lysate
Lane 7: U251 cell lysate
Lane 8: Mouse lung lysate
Lane 9: Rat brain lysate
Lane 10: Rat testis lysate
Lane11: Rat lung lysate

Ссылки

Даташит

Anti-Human YWHAG Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

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