Anti-Human ZRANB3 Polyclonal Antibody (HV828014)

Name: Anti-Human ZRANB3 Polyclonal Antibody
Brand: abinScience
SKU: HV828014
Price: 168 USD
Availability: InStock

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Spécification :

50ug
100ug

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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HV828014

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human ZRANB3 (Asp27-Arg256).

Cible

Annealing helicase 2, ZRANB3, Zinc finger Ran-binding domain-containing protein 3, AH2, DNA annealing helicase and endonuclease ZRANB3

Purification

Purified by antigen affinity column.

Numéro d'accès

Q5FWF4

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

DNA annealing helicase and endonuclease ZRANB3 is a ~123 kDa protein. DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events. Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase. In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks.

1. Yusufzai, T. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 20970-3. PMID: 21078962

2. Ciccia, A. et al. (2012) Molecular cell 47, 396-409. PMID: 22704558

3. Yuan, J. et al. (2012) Molecular cell 47, 410-21. PMID: 22705370

4. Weston, R. et al. (2012) Genes & development 26, 1558-72. PMID: 22759634

5. Badu-Nkansah, A. et al. (2016) The Journal of biological chemistry 291, 8251-7. PMID: 26884333

6. Sebesta, M. et al. (2017) Nature communications 8, 15847. PMID: 28621305

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human ZRANB3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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