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| カタログ番号 | HF907014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human ZWINT Polyclonal Antibody (HF907014) is a rabbit polyclonal antibody detecting ZWINT in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human ZWINT (Met1-Pro277). | ||||||||
| ターゲット | ZWINT | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | O95229 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 別名 | ZW10 interactor, ZW10-interacting protein 1, Zwint-1, ZWINT | ||||||||
| 背景 | Outer kinetochore KNL1 complex subunit ZWINT is a ~31 kDa protein. Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions. Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules. The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle. Targets the RZZ complex to the kinetochore at prometaphase. Recruits MAD2L1 to the kinetochore, but is not required for BUB1B localization. 1. Musio, A. et al. (2004) Gene 331, 33-40. PMID: 15094189 2. Wang, H. et al. (2004) The Journal of biological chemistry 279, 54590-8. PMID: 15485811 3. Kops, GJ. et al. (2005) The Journal of cell biology 169, 49-60. PMID: 15824131 4. Lin, YT. et al. (2006) Oncogene 25, 6901-14. PMID: 16732327 5. Petrovic, A. et al. (2014) Molecular cell 53, 591-605. PMID: 24530301 6. Petrovic, A. et al. (2016) Cell 167, 1028-1040.e15. PMID: 27881301 7. Yatskevich, S. et al. (2024) Nature structural & molecular biology 31, 874-883. PMID: 38459127 8. Polley, S. et al. (2024) Nature structural & molecular biology 31, 861-873. PMID: 38459128 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-ZWINT polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: SW620 cell lysate
Lane 3: 293T cell lysate
Lane 4: Jurkat cell lysate
Lane 5: Hela cell lysate
Lane 6: Raji cell lysate
Lane 7: K562 cell lysate
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