Anti-Hypusine Recombinant Antibody (Hpu24) (YP587013)

Name: Anti-Hypusine Recombinant Antibody (Hpu24)
Brand: abinScience
SKU: YP587013
Price: 126 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

YP587013

Reactividad de especies

General

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Monoclonal

Isotipo

IgG

ID de clon

Hpu24

Objetivo

Hypusine, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D, EIF5A

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

PubChem: 65396

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Referencias

1. Shiba T,et al. Hypusine, a new amino acid occurring in bovine brain. Isolation and structural determination. Biochim Biophys Acta. 1971 Sep 21;244(3):523-31. [YP587013]
2. ark MH, et al. Hypusine, a polyamine-derived amino acid critical for eukaryotic translation. J Biol Chem. 2018 Nov 30;293(48):18710-18718. [YP587013]

Nota

For research use only.

Imágenes

Bioactivity

SEC-HPLC detection for Anti-Hypusine antibody (Hpu24).
SDS-PAGE

SDS-PAGE for Anti-Hypusine antibody (Hpu24).
Western blot

Western blot analysis was performed using anti-EIF5A monoclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
Lane 3: 293T cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: Mouse pancears lysate
Lane 7: Rat pancears lysate
Western blot

Western blot analysis was performed using anti-Eif5a polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: K562 cell lysate
Lane 4: PC12 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: MCF7 cell lysate
Lane 7: 293T cell lysate