Anti-Hypusine Recombinant Antibody (Hpu24) (YP587013)

Name: Anti-Hypusine Recombinant Antibody (Hpu24)
Brand: abinScience
SKU: YP587013
Price: 126 USD
Availability: InStock

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20ug
50ug
100ug
1mg

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

YP587013

Reactivité des espèces

General

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Monoclonal

Isotype

IgG

ID de clone

Hpu24

Cible

Hypusine, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D, EIF5A

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

PubChem: 65396

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Reférences

1. Shiba T,et al. Hypusine, a new amino acid occurring in bovine brain. Isolation and structural determination. Biochim Biophys Acta. 1971 Sep 21;244(3):523-31. [YP587013]
2. ark MH, et al. Hypusine, a polyamine-derived amino acid critical for eukaryotic translation. J Biol Chem. 2018 Nov 30;293(48):18710-18718. [YP587013]

Note

For research use only.

Images

Bioactivity

SEC-HPLC detection for Anti-Hypusine antibody (Hpu24).
SDS-PAGE

SDS-PAGE for Anti-Hypusine antibody (Hpu24).
Western blot

Western blot analysis was performed using anti-EIF5A monoclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
Lane 3: 293T cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: Mouse pancears lysate
Lane 7: Rat pancears lysate
Western blot

Western blot analysis was performed using anti-Eif5a polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: K562 cell lysate
Lane 4: PC12 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: MCF7 cell lysate
Lane 7: 293T cell lysate