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| カタログ番号 | YP587013 | ||||||
|---|---|---|---|---|---|---|---|
| 種反応性 | General | ||||||
| アプリケーション | ELISA, IHC, WB | ||||||
| 宿主種 | Rabbit | ||||||
| クローン性 | Monoclonal | ||||||
| アイソタイプ | IgG | ||||||
| クローンID | Hpu24 | ||||||
| ターゲット | Hypusine, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D, EIF5A | ||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||
| 純度 | >95% as determined by SDS-PAGE. | ||||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||||
| アクセッション番号 | PubChem: 65396 | ||||||
| 形態 | Liquid | ||||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| 参考文献 | 1. Shiba T,et al. Hypusine, a new amino acid occurring in bovine brain. Isolation and structural determination. Biochim Biophys Acta. 1971 Sep 21;244(3):523-31. [YP587013] 2. ark MH, et al. Hypusine, a polyamine-derived amino acid critical for eukaryotic translation. J Biol Chem. 2018 Nov 30;293(48):18710-18718. [YP587013] |
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| 注意事項 | For research use only. |
SEC-HPLC detection for Anti-Hypusine antibody (Hpu24).
SDS-PAGE for Anti-Hypusine antibody (Hpu24).
Western blot analysis was performed using anti-EIF5A monoclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
Lane 3: 293T cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: Mouse pancears lysate
Lane 7: Rat pancears lysate
Western blot analysis was performed using anti-Eif5a polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: K562 cell lysate
Lane 4: PC12 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: MCF7 cell lysate
Lane 7: 293T cell lysate
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