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Anti-Human IL-7 Polyclonal Antibody (HY408014)

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Vista general
Número de catálogoHY408014
Descripción
Anti-IL7 Polyclonal Antibody (HY408014) is a rabbit polyclonal antibody detecting IL7 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human IL7 (Asp26-His177).
Objetivo IL-7, Interleukin-7, IL7
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >95% as determined by SDS-PAGE.
Purificación Purified by antigen affinity column.
Número de acceso P13232
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Interleukin-7 (IL7) is a ~20 kDa protein. Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis. Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG. Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5.

1. Bazdar, DA. et al. (2015) Journal of immunology (Baltimore, Md. : 1950) 194, 4698-704. PMID: 25870237
2. Dittel, BN. et al. (1995) Journal of immunology (Baltimore, Md. : 1950) 154, 58-67. PMID: 7527823
3. Kondo, M. et al. (1994) Science (New York, N.Y.) 263, 1453-4. PMID: 8128231
4. Johnson, SE. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 180, 8109-17. PMID: 18523275
5. Rochman, Y. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 19455-60. PMID: 20974963
Nota For research use only.
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Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human IL-7 Polyclonal Antibody

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