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Обзор
Каталожный номерHY408014
Описание
Anti-IL7 Polyclonal Antibody (HY408014) is a rabbit polyclonal antibody detecting IL7 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human IL7 (Asp26-His177).
Цель IL-7, Interleukin-7, IL7
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >95% as determined by SDS-PAGE.
Очистка Purified by antigen affinity column.
Номер доступа P13232
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Interleukin-7 (IL7) is a ~20 kDa protein. Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis. Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG. Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5.

1. Bazdar, DA. et al. (2015) Journal of immunology (Baltimore, Md. : 1950) 194, 4698-704. PMID: 25870237
2. Dittel, BN. et al. (1995) Journal of immunology (Baltimore, Md. : 1950) 154, 58-67. PMID: 7527823
3. Kondo, M. et al. (1994) Science (New York, N.Y.) 263, 1453-4. PMID: 8128231
4. Johnson, SE. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 180, 8109-17. PMID: 18523275
5. Rochman, Y. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 19455-60. PMID: 20974963
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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