Anti-ILK Polyclonal Antibody (HC502014)

Name: Anti-ILK Polyclonal Antibody
Brand: abinScience
SKU: HC502014
Price: 168 USD
Availability: InStock

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50ug
100ug

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Aperçu

Numéro de catalogue

HC502014

Description

Anti-ILK Polyclonal Antibody (HC502014) is a rabbit polyclonal antibody detecting ILK in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human ILK (Asn183-Lys452).

Cible

ILK, ILK2, ILK1, ILK-2, ILK-1, 59 kDa serine/threonine-protein kinase, Beta-integrin-linked kinase, p59ILK, Integrin-linked protein kinase

Purification

Purified by antigen affinity column.

Numéro d'accès

Q13418

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Scaffold protein ILK is a ~51 kDa protein. Scaffold protein which mediates protein-protein interactions during a range of cellular events including focal adhesion assembly, cell adhesion and cell migration. Regulates integrin-mediated signal transduction by contributing to inside-out integrin activation. Recruits PARVA and LIMS1/PITCH to form the heterotrimeric IPP (ILK-PINCH-PARVIN) complex which binds to F-actin via the C-terminal tail of LIMS1 and the N-terminal region of PARVA, promoting F-actin filament bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration. Binding to PARVA promotes effective assembly of ILK into focal adhesions while PARVA-bound ILK can simultaneously engage integrin-beta cytoplasmic tails to mediate cell adhesion. Plays a role with PARVG in promoting the cell adhesion and spreading of leukocytes.

1. Acconcia, F. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 6782-7. PMID: 17420447

2. Fukuda, K. et al. (2009) Molecular cell 36, 819-30. PMID: 20005845

3. Vaynberg, J. et al. (2018) Nature communications 9, 4465. PMID: 30367047

4. Yang, L. et al. (2020) Nature 583, 133-138. PMID: 32528174

5. Yoshimi, R. et al. (2006) Journal of immunology (Baltimore, Md. : 1950) 176, 3611-24. PMID: 16517730

6. Delcommenne, M. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 11211-6. PMID: 9736715

7. Fielding, AB. et al. (2008) The Journal of cell biology 180, 681-9. PMID: 18283114

Note

For research use only.

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Reférences

Fiche technique

Anti-ILK Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-ILK Polyclonal Antibody (HC502014)

Aperçu

Images

Reférences

Fiche technique

SDS