Anti-IRS1 Polyclonal Antibody (HW428014)

Name: Anti-IRS1 Polyclonal Antibody
Brand: abinScience
SKU: HW428014
Price: 168 USD
Availability: InStock

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Especificación:

50ug
100ug

Número:

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Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HW428014

Descripción

Anti-IRS1 Polyclonal Antibody (HW428014) is a rabbit polyclonal antibody detecting IRS1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human IRS1 (Pro4-Arg267).

Objetivo

IRS-1, IRS1, Insulin receptor substrate 1

Purificación

Purified by antigen affinity column.

Número de acceso

P35568

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Insulin receptor substrate 1 (IRS1) is a ~131 kDa protein. Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R. Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism. Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2. Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade. Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation.

1. Craparo, A. et al. (1995) The Journal of biological chemistry 270, 15639-43. PMID: 7541045

2. Yu, W. et al. (2021) The Journal of biological chemistry 296, 100739. PMID: 33991522

3. Woo, JR. et al. (2024) Proceedings of the National Academy of Sciences of the United States of America 121, e2401716121. PMID: 38625937

4. Longobardi, L. et al. (2009) Growth factors (Chur, Switzerland) 27, 309-20. PMID: 19639489

5. Tu, Y. et al. (2001) The Biochemical journal 354, 315-22. PMID: 11171109

6. Lee, CH. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 11713-7. PMID: 8265614

7. Geng, Y. et al. (2014) The Journal of biological chemistry 289, 11230-11241. PMID: 24616100

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-IRS1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo