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| カタログ番号 | HW428014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-IRS1 Polyclonal Antibody (HW428014) is a rabbit polyclonal antibody detecting IRS1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Xenopus laevis.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human IRS1 (Pro4-Arg267). | ||||||||
| ターゲット | IRS-1, IRS1, Insulin receptor substrate 1 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P35568 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Insulin receptor substrate 1 (IRS1) is a ~131 kDa protein. Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R. Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism. Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2. Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade. Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation. 1. Craparo, A. et al. (1995) The Journal of biological chemistry 270, 15639-43. PMID: 7541045 2. Yu, W. et al. (2021) The Journal of biological chemistry 296, 100739. PMID: 33991522 3. Woo, JR. et al. (2024) Proceedings of the National Academy of Sciences of the United States of America 121, e2401716121. PMID: 38625937 4. Longobardi, L. et al. (2009) Growth factors (Chur, Switzerland) 27, 309-20. PMID: 19639489 5. Tu, Y. et al. (2001) The Biochemical journal 354, 315-22. PMID: 11171109 6. Lee, CH. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 11713-7. PMID: 8265614 7. Geng, Y. et al. (2014) The Journal of biological chemistry 289, 11230-11241. PMID: 24616100 | ||||||||
| 注意事項 | For research use only. |
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