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Anti-IYD Polyclonal Antibody (HB478014)

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Vista general
Número de catálogoHB478014
Descripción
Anti-IYD Polyclonal Antibody (HB478014) is a rabbit polyclonal antibody detecting Iodotyrosine deiodinase 1; IYD-1; 1.21.1.1; Iodotyrosine dehalogenase 1; IYD; C6orf71; DEHAL1 in ELISA, IHC, WB. Suitable for Human, Rat, Mouse, and Pig.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant human IYD (Tyr81-Val289).
Objetivo Iodotyrosine deiodinase 1; IYD-1; 1.21.1.1; Iodotyrosine dehalogenase 1; IYD; C6orf71; DEHAL1
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q6PHW0, Q5BK17, Q9DCX8, Q6TA49
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Iodotyrosine deiodinase 1 (IYD) is a ~33 kDa protein. Catalyzes the dehalogenation of halotyrosines such as 3-bromo-L-tyrosine, 3-chloro-L-tyrosine, 3-iodo-L-tyrosine and 3,5-diiodo-L-tyrosine. During thyroid hormone biosynthesis, facilitates iodide salvage by catalysing the oxidative NADPH-dependent deiodination of the halogenated by-products of thyroid hormone production, monoiodotyrosine (L-MIT) and diiodotyrosine (L-DIT). The scavanged iodide can then reenter the hormone-producing pathways. Acts more efficiently on 3-iodo-L-tyrosine than 3,5-diiodo-L-tyrosine.

1. Gnidehou, S. et al. (2004) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 18, 1574-6. PMID: 15289438
2. Moreno, JC. et al. (2008) The New England journal of medicine 358, 1811-8. PMID: 18434651
3. Hu, J. et al. (2015) The Journal of biological chemistry 290, 590-600. PMID: 25395621
4. Ingavat, N. et al. (2017) Biochemistry 56, 1130-1139. PMID: 28157283
Nota For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-IYD Polyclonal Antibody

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