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| カタログ番号 | HV546014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-KISS1R Polyclonal Antibody (HV546014) is a rabbit polyclonal antibody detecting KISS1R in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human KISS1R (His2-Trp43). | ||||||||
| ターゲット | KiSS-1R, G-protein coupled receptor 54, KiSS-1 receptor, GPR54, KISS1R, hOT7T175, G-protein coupled receptor OT7T175, Metastin receptor, Hypogonadotropin-1, AXOR12, Kisspeptins receptor | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q969F8 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | KiSS-1 receptor (KISS1R) is a ~42 kDa protein. Receptor for kisspeptins (kisspeptin-10, kisspeptin-13, kisspeptin-14 and metastin/kisspeptin-54). The hypothalamic KISS1/KISS1R signaling system plays a central role in the regulation of the hypothalamic-pituitary-gonadal reproductive axis by modulating the secretion of gonadotropin-releasing hormone (GnRH) from GnRH neurons. In these neurons, kisspeptin binding to its receptor activates G(q)-dependent signaling, leading to phospholipase C (PLC) activation, and hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2). The subsequent rise in intracellular calcium levels results in the inhibition of inward rectifier potassium channels and activation of TRPC-like cation channels, leading to GnRH neurons depolarization and stimulation. In addition to this pathway, kisspeptin also triggers G(q)-independent signaling via beta-arrestin, leading to MAPK cascade activation and ERK1/ERK2 phosphorylation. 1. Kotani, M. et al. (2001) The Journal of biological chemistry 276, 34631-6. PMID: 11457843 2. Hori, A. et al. (2001) Biochemical and biophysical research communications 286, 958-63. PMID: 11527393 3. Bilban, M. et al. (2004) Journal of cell science 117, 1319-28. PMID: 15020672 4. Becker, JA. et al. (2005) Biochemical and biophysical research communications 326, 677-86. PMID: 15596153 5. de Roux, N. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 10972-6. PMID: 12944565 6. Seminara, SB. et al. (2003) The New England journal of medicine 349, 1614-27. PMID: 14573733 7. Semple, RK. et al. (2005) The Journal of clinical endocrinology and metabolism 90, 1849-55. PMID: 15598687 8. Tenenbaum-Rakover, Y. et al. (2007) The Journal of clinical endocrinology and metabolism 92, 1137-44. PMID: 17164310 9. Teles, MG. et al. (2008) The New England journal of medicine 358, 709-15. PMID: 18272894 | ||||||||
| 注意事項 | For research use only. |
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