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Anti-KMO Polyclonal Antibody (HT083014)

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Aperçu
Numéro de catalogueHT083014
Description
Anti-KMO Polyclonal Antibody (HT083014) is a rabbit polyclonal antibody detecting KMO in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human KMO (Met1-Glu366).
Cible KMO, Kynurenine 3-hydroxylase, Kynurenine 3-monooxygenase
Purification Purified by antigen affinity column.
Numéro d'accès O15229
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Kynurenine 3-monooxygenase (KMO) is a ~55 kDa protein. Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract (Probable).

1. Amaral, M. et al. (2013) Nature 496, 382-5. PMID: 23575632
2. Mole, DJ. et al. (2016) Nature medicine 22, 202-9. PMID: 26752518
3. Hutchinson, JP. et al. (2017) Nature communications 8, 15827. PMID: 28604669
4. Gao, J. et al. (2018) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 32, 2036-2045. PMID: 29208702
5. Kim, HT. et al. (2018) Cell chemical biology 25, 426-438.e4. PMID: 29429898
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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