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Anti-KRT18 Polyclonal Antibody (HY198014)

Anti-KRT18 Polyclonal Antibody
Anti-KRT18 Polyclonal Antibody
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  • 20ug
  • 50ug
  • 100ug
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Vista general
Número de catálogoHY198014
Descripción
Anti-KRT18 Polyclonal Antibody (HY198014) is a rabbit polyclonal antibody detecting KRT18 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human KRT18 (Asp238-Leu396).
Objetivo Cytokeratin-18, CYK18, K18, Keratin, type I cytoskeletal 18, KRT18, CK-18, Keratin-18, Cell proliferation-inducing gene 46 protein
Purificación Purified by antigen affinity column.
Número de acceso P05783
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Keratin, type I cytoskeletal 18 (KRT18) is a ~48 kDa protein. Required for the formation of KRT8/KRT18 filaments that are involved in ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber formation and reinforcement. Also acts downstream of ROCK kinase activation as part of a positive feedback mechanism in response to cellular mechanical stress loading. Organization and orientation of KRT18 filaments are responsible for the properly elongated morphology of epithelial tubules. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization.

1. Fujiwara, S. et al. (2016) Molecular biology of the cell 27, 954-66. PMID: 26823019
Nota For research use only.
Imágenes
  • Anti-KRT18 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-KRT18 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HT-29 cell lysate
    Lane 2: Hela cell lysate
    Lane 3: A431 cell lysate
    Lane 4: K562 cell lysate

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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