Anti-LC3B Polyclonal Antibody (HV519014)

Name: Anti-LC3B Polyclonal Antibody
Brand: abinScience
SKU: HV519014
Price: 96 USD
Availability: InStock

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概要
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参考文献
データシート
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概要

カタログ番号

HV519014

説明

Anti-LC3B Polyclonal Antibody (HV519014) is a rabbit polyclonal antibody detecting MAP1LC3B in ELISA, IHC, WB. Suitable for Human, Bovine, Mouse, Rat, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human LC3B (Met1-Gly120).

ターゲット

Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1LC3B, MAP1ALC3

エンドトキシンレベル

Please contact with the lab for this information.

精製

Purified by antigen affinity column.

アクセッション番号

Q9GZQ8, A6NCE7, O41515, Q9CQV6, Q62625

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Microtubule-associated protein 1 light chain 3 beta (LC3B/MAP1LC3B) is a ~14 kDa protein. Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria. While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

1. Weidberg, H. et al. (2010) The EMBO journal 29, 1792-802. PMID: 20418806

2. Zhu, Y. et al. (2013) The Journal of biological chemistry 288, 1099-113. PMID: 23209295

3. Wei, Y. et al. (2017) Cell 168, 224-238.e10. PMID: 28017329

4. Sentelle, RD. et al. (2012) Nature chemical biology 8, 831-8. PMID: 22922758

5. Tang, Z. et al. (2013) Nature 502, 254-7. PMID: 24089205

6. An, H. et al. (2019) Molecular cell 74, 891-908.e10. PMID: 31006537

7. Chino, H. et al. (2019) Molecular cell 74, 909-921.e6. PMID: 31006538

注意事項

For research use only.

イメージ

参考文献

データシート

Anti-LC3B Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-LC3B Polyclonal Antibody (HV519014)

概要

イメージ

参考文献

データシート

SDS

クイックアクセス

お問い合わせ