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Anti-LEF1 Polyclonal Antibody (HV355014)

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Anti-LEF1 Polyclonal Antibody
Anti-LEF1 Polyclonal Antibody
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Vista general
Número de catálogoHV355014
Descripción
Anti-LEF1 Polyclonal Antibody (HV355014) is a rabbit polyclonal antibody detecting LEF1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human LEF1 (Glu45-His260).
Objetivo Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, LEF-1, TCF1-alpha, LEF1
Purificación Purified by antigen affinity column.
Número de acceso Q9UJU2
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Lymphoid enhancer-binding factor 1 (LEF1) is a ~44 kDa protein. Transcription factor that binds DNA in a sequence-specific manner. Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1.

1. Waterman, ML. et al. (1991) Genes & development 5, 656-69. PMID: 2010090
2. Brantjes, H. et al. (2001) Nucleic acids research 29, 1410-9. PMID: 11266540
3. Jesse, S. et al. (2010) International journal of cancer 126, 1109-20. PMID: 19653274
Nota For research use only.
Imágenes
  • Anti-LEF1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-LEF1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Jurkat cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: BxPC3 cell lysate
    Lane 4: K562 cell lysate
    Lane 5: Mouse thymus lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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