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Anti-LSS Polyclonal Antibody (HW356014)

Anti-LSS Polyclonal Antibody
Anti-LSS Polyclonal Antibody
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Aperçu
Numéro de catalogueHW356014
Description
Anti-LSS Polyclonal Antibody (HW356014) is a rabbit polyclonal antibody detecting LSS in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human LSS (Asp388-Pro549).
Cible OSC, Lanosterol synthase, hOSC, Oxidosqualene--lanosterol cyclase, 2, 3-epoxysqualene--lanosterol cyclase, LSS
Purification Purified by antigen affinity column.
Numéro d'accès P48449
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Lanosterol synthase (LSS) is a ~83 kDa protein. Key enzyme in the cholesterol biosynthesis pathway. Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. Through the production of lanosterol may regulate lens protein aggregation and increase transparency.

1. Ruf, A. et al. (2004) Biochemical and biophysical research communications 315, 247-54. PMID: 14766201
2. Zhao, L. et al. (2015) Nature 523, 607-11. PMID: 26200341
3. Baker, CH. et al. (1995) Biochemical and biophysical research communications 213, 154-60. PMID: 7639730
Note For research use only.
Images
  • Anti-LSS Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-LSS polyclonal antibody at 1ug/mL on various samples.
    Lane 1: SH-SY5Y cell lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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