Anti-MACROH2A1 Polyclonal Antibody (HF633014)

Name: Anti-MACROH2A1 Polyclonal Antibody
Brand: abinScience
SKU: HF633014
Price: 96 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HF633014

Description

Anti-MACROH2A1 Polyclonal Antibody (HF633014) is a rabbit polyclonal antibody detecting MACROH2A1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human MACROH2A1 (Ile109-Leu369).

Cible

Histone macroH2A1, mH2A1, H2A/y, MACROH2A1, Medulloblastoma antigen MU-MB-50.205, Core histone macro-H2A.1, H2AFY, Histone H2A.y

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

O75367

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Core histone macro-H2A.1 (MACROH2A1) is a ~39 kDa protein. Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation.

1. Angelov, D. et al. (2003) Molecular cell 11, 1033-41. PMID: 12718888

2. Zhang, R. et al. (2005) Developmental cell 8, 19-30. PMID: 15621527

3. Doyen, CM. et al. (2006) Molecular and cellular biology 26, 1156-64. PMID: 16428466

4. Hernández-Muñoz, I. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 7635-40. PMID: 15897469

5. Chakravarthy, S. et al. (2005) Molecular and cellular biology 25, 7616-24. PMID: 16107708

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MACROH1A2 polyclonal antibody at 1μg/ml on various samples.
Lane 1: MCF7 cell lysate
Lane 2: Hela cell lysate
Lane 3: Rat brain lysate
Lane 4: Rat liver lysate
Lane 5: 293T cell lysate
Lane 6: HepG2 cell lysate
Lane 7: Mouse brain lysate
Lane 8: Mouse liver lysate
Lane 9: Rat lung lysate