Anti-MAPK8 Polyclonal Antibody (HW707014)

Name: Anti-MAPK8 Polyclonal Antibody
Brand: abinScience
SKU: HW707014
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
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概要

カタログ番号

HW707014

説明

Anti-MAPK8 Polyclonal Antibody (HW707014) is a rabbit polyclonal antibody detecting MAPK8 in ELISA, IHC, WB. Suitable for Cyprinus carpio, Danio rerio, Chicken, Human, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human MAPK8 (Tyr26-Ile321).

ターゲット

MAP kinase 8, JNK1, SAPK1C, Stress-activated protein kinase 1c, Mitogen-activated protein kinase 8, SAPK1, c-Jun N-terminal kinase 1, SAPK1c, MAPK8, Stress-activated protein kinase JNK1, PRKM8, JNK-46, MAPK 8

精製

Purified by antigen affinity column.

アクセッション番号

P45983

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Mitogen-activated protein kinase 8 (MAPK8) is a ~48 kDa protein. Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins.

1. Song, N. et al. (2017) Molecular cell 68, 185-197.e6. PMID: 28943315

2. Murata, T. et al. (2008) Analytical biochemistry 376, 115-21. PMID: 18307971

3. Miotto, B. et al. (2011) Molecular cell 44, 62-71. PMID: 21856198

4. Tomlinson, V. et al. (2010) Cell death & disease 1, e29. PMID: 21364637

5. Deng, H. et al. (2011) The international journal of biochemistry & cell biology 43, 409-15. PMID: 21095239

6. Wei, Y. et al. (2008) Molecular cell 30, 678-88. PMID: 18570871

7. Hu, Y. et al. (2006) Molecular and cellular biology 26, 3282-94. PMID: 16581800

8. Zhang, L. et al. (2007) Molecular and cellular biology 27, 2861-9. PMID: 17296730

9. Nasrin, N. et al. (2009) PloS one 4, e8414. PMID: 20027304

10. Yoshitane, H. et al. (2012) EMBO reports 13, 455-61. PMID: 22441692

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-JNK polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate

参考文献

データシート

Anti-MAPK8 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

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アッセイキット

Anti-MAPK8 Polyclonal Antibody (HW707014)

概要

イメージ

参考文献

データシート

SDS

クイックアクセス

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