Anti-MAPK8 Polyclonal Antibody (HW707014)

Name: Anti-MAPK8 Polyclonal Antibody
Brand: abinScience
SKU: HW707014
Price: 96 USD
Availability: InStock

Даташит Поделиться продуктом Копировать информацию

Цена(USD):

Спецификация:

20ug
50ug
100ug

Количество:

Свяжитесь с нами

Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HW707014

Описание

Anti-MAPK8 Polyclonal Antibody (HW707014) is a rabbit polyclonal antibody detecting MAPK8 in ELISA, IHC, WB. Suitable for Cyprinus carpio, Danio rerio, Chicken, Human, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human MAPK8 (Tyr26-Ile321).

Цель

MAP kinase 8, JNK1, SAPK1C, Stress-activated protein kinase 1c, Mitogen-activated protein kinase 8, SAPK1, c-Jun N-terminal kinase 1, SAPK1c, MAPK8, Stress-activated protein kinase JNK1, PRKM8, JNK-46, MAPK 8

Очистка

Purified by antigen affinity column.

Номер доступа

P45983

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Mitogen-activated protein kinase 8 (MAPK8) is a ~48 kDa protein. Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins.

1. Song, N. et al. (2017) Molecular cell 68, 185-197.e6. PMID: 28943315

2. Murata, T. et al. (2008) Analytical biochemistry 376, 115-21. PMID: 18307971

3. Miotto, B. et al. (2011) Molecular cell 44, 62-71. PMID: 21856198

4. Tomlinson, V. et al. (2010) Cell death & disease 1, e29. PMID: 21364637

5. Deng, H. et al. (2011) The international journal of biochemistry & cell biology 43, 409-15. PMID: 21095239

6. Wei, Y. et al. (2008) Molecular cell 30, 678-88. PMID: 18570871

7. Hu, Y. et al. (2006) Molecular and cellular biology 26, 3282-94. PMID: 16581800

8. Zhang, L. et al. (2007) Molecular and cellular biology 27, 2861-9. PMID: 17296730

9. Nasrin, N. et al. (2009) PloS one 4, e8414. PMID: 20027304

10. Yoshitane, H. et al. (2012) EMBO reports 13, 455-61. PMID: 22441692

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-JNK polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate

Ссылки

Даташит

Anti-MAPK8 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа