Anti-MBD2 Polyclonal Antibody (HV150014)

Name: Anti-MBD2 Polyclonal Antibody
Brand: abinScience
SKU: HV150014
Price: 96 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HV150014

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human MBD2 (Glu145-Ala411).

Objetivo

Methyl-CpG-binding domain protein 2, Methyl-CpG-binding protein MBD2, MBD2, DMTase, Demethylase

Purificación

Purified by antigen affinity column.

Número de acceso

Q9UBB5

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Methyl-CpG-binding domain protein 2 (MBD2) is a ~43 kDa protein. Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases to chromatin. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Acts as a transcriptional repressor and plays a role in gene silencing.

1. Hendrich, B. et al. (1998) Molecular and cellular biology 18, 6538-47. PMID: 9774669

2. Tatematsu, KI. et al. (2000) Genes to cells : devoted to molecular & cellular mechanisms 5, 677-88. PMID: 10947852

3. Cramer, JM. et al. (2014) The Journal of biological chemistry 289, 1294-302. PMID: 24307175

4. Ng, HH. et al. (1999) Nature genetics 23, 58-61. PMID: 10471499

5. Le Guezennec, X. et al. (2006) Molecular and cellular biology 26, 843-51. PMID: 16428440

6. Hoffmeister, H. et al. (2017) Nucleic acids research 45, 10534-10554. PMID: 28977666

7. Brackertz, M. et al. (2006) Nucleic acids research 34, 397-406. PMID: 16415179

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-MBD2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate

Referencias

Datasheet

Anti-MBD2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo