Anti-MBD2 Polyclonal Antibody (HV150014)

Name: Anti-MBD2 Polyclonal Antibody
Brand: abinScience
SKU: HV150014
Price: 96 USD
Availability: InStock

Fiche technique Partager le produit Copier les informations

Prix(USD) :

Spécification :

20ug
50ug
100ug

Nombre :

Contactez-nous

Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HV150014

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human MBD2 (Glu145-Ala411).

Cible

Methyl-CpG-binding domain protein 2, Methyl-CpG-binding protein MBD2, MBD2, DMTase, Demethylase

Purification

Purified by antigen affinity column.

Numéro d'accès

Q9UBB5

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Methyl-CpG-binding domain protein 2 (MBD2) is a ~43 kDa protein. Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases to chromatin. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Acts as a transcriptional repressor and plays a role in gene silencing.

1. Hendrich, B. et al. (1998) Molecular and cellular biology 18, 6538-47. PMID: 9774669

2. Tatematsu, KI. et al. (2000) Genes to cells : devoted to molecular & cellular mechanisms 5, 677-88. PMID: 10947852

3. Cramer, JM. et al. (2014) The Journal of biological chemistry 289, 1294-302. PMID: 24307175

4. Ng, HH. et al. (1999) Nature genetics 23, 58-61. PMID: 10471499

5. Le Guezennec, X. et al. (2006) Molecular and cellular biology 26, 843-51. PMID: 16428440

6. Hoffmeister, H. et al. (2017) Nucleic acids research 45, 10534-10554. PMID: 28977666

7. Brackertz, M. et al. (2006) Nucleic acids research 34, 397-406. PMID: 16415179

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MBD2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate

Reférences

Fiche technique

Anti-MBD2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai