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| カタログ番号 | HV150014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human MBD2 (Glu145-Ala411). | ||||||||
| ターゲット | Methyl-CpG-binding domain protein 2, Methyl-CpG-binding protein MBD2, MBD2, DMTase, Demethylase | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q9UBB5 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Methyl-CpG-binding domain protein 2 (MBD2) is a ~43 kDa protein. Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases to chromatin. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Acts as a transcriptional repressor and plays a role in gene silencing. 1. Hendrich, B. et al. (1998) Molecular and cellular biology 18, 6538-47. PMID: 9774669 2. Tatematsu, KI. et al. (2000) Genes to cells : devoted to molecular & cellular mechanisms 5, 677-88. PMID: 10947852 3. Cramer, JM. et al. (2014) The Journal of biological chemistry 289, 1294-302. PMID: 24307175 4. Ng, HH. et al. (1999) Nature genetics 23, 58-61. PMID: 10471499 5. Le Guezennec, X. et al. (2006) Molecular and cellular biology 26, 843-51. PMID: 16428440 6. Hoffmeister, H. et al. (2017) Nucleic acids research 45, 10534-10554. PMID: 28977666 7. Brackertz, M. et al. (2006) Nucleic acids research 34, 397-406. PMID: 16415179 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-MBD2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
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