Anti-MBD2 Polyclonal Antibody (HV150014)

Name: Anti-MBD2 Polyclonal Antibody
Brand: abinScience
SKU: HV150014
Price: 96 USD
Availability: InStock

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HV150014

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human MBD2 (Glu145-Ala411).

Цель

Methyl-CpG-binding domain protein 2, Methyl-CpG-binding protein MBD2, MBD2, DMTase, Demethylase

Очистка

Purified by antigen affinity column.

Номер доступа

Q9UBB5

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Methyl-CpG-binding domain protein 2 (MBD2) is a ~43 kDa protein. Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases to chromatin. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Acts as a transcriptional repressor and plays a role in gene silencing.

1. Hendrich, B. et al. (1998) Molecular and cellular biology 18, 6538-47. PMID: 9774669

2. Tatematsu, KI. et al. (2000) Genes to cells : devoted to molecular & cellular mechanisms 5, 677-88. PMID: 10947852

3. Cramer, JM. et al. (2014) The Journal of biological chemistry 289, 1294-302. PMID: 24307175

4. Ng, HH. et al. (1999) Nature genetics 23, 58-61. PMID: 10471499

5. Le Guezennec, X. et al. (2006) Molecular and cellular biology 26, 843-51. PMID: 16428440

6. Hoffmeister, H. et al. (2017) Nucleic acids research 45, 10534-10554. PMID: 28977666

7. Brackertz, M. et al. (2006) Nucleic acids research 34, 397-406. PMID: 16415179

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-MBD2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate

Ссылки

Даташит

Anti-MBD2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

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