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| カタログ番号 | HW401014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-MCM2 Polyclonal Antibody (HW401014) is a rabbit polyclonal antibody detecting MCM2 in ELISA, IHC, WB. Suitable for Human, Mouse, Xenopus laevis, and Xenopus tropicalis.
Highlights
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| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human MCM2 (Phe657-Phe904). | ||||||||
| ターゲット | CCNL1, KIAA0030, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, MCM2, CDCL1, BM28, DNA replication licensing factor MCM2 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P49736 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | DNA replication licensing factor MCM2 is a ~101 kDa protein. Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. 1. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425 2. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004 3. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328 4. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232 5. Todorov, IT. et al. (1994) Journal of cell science 107 ( Pt 1), 253-65. PMID: 8175912 6. Gao, J. et al. (2015) PloS one 10, e0133522. PMID: 26196677 | ||||||||
| 注意事項 | For research use only. |
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