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Anti-MERS-CoV ORF4a Polyclonal Antibody (VK073054)

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Vista general
Número de catálogoVK073054
Descripción
Anti-MERS-CoV ORF4a Polyclonal Antibody (VK073054) is a rabbit polyclonal antibody detecting Non-structural protein ORF4a; ORF4a; ORF4a in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesMiddle East respiratory syndrome-related coronavirus (MERS-CoV)
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant mers-cov ORF4a (Met1-Asn109).
Objetivo Non-structural protein ORF4a; ORF4a; ORF4a
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso K9N4V0
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Non-structural protein ORF4a is a ~12 kDa protein. DsRNA-binding protein that plays a role in the inhibition of host innate response. Suppresses host PACT-induced activation of RIGI and MDA5 and thereby circumvents the production of type I interferons. Also prevents the activation of host NF-kappa-B. Inhibits the integrated stress response (ISR) in the infected cell by binding to dsRNA and inhibiting EIF2AK2-mediated phosphorylation of EIF2S1/eIF2-alpha. Stress granule formation is thus inhibited, which allows protein synthesis and viral replication.

1. Niemeyer, D. et al. (2013) Journal of virology 87, 12489-95. PMID: 24027320
2. Yang, Y. et al. (2013) Protein & cell 4, 951-61. PMID: 24318862
3. Siu, KL. et al. (2014) Journal of virology 88, 4866-76. PMID: 24522921
4. Nakagawa, K. et al. (2018) Journal of virology 92. PMID: 30068649
Nota For research use only
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Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-MERS-CoV ORF4a Polyclonal Antibody

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