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Anti-MGAM/MGA Polyclonal Antibody (HT381014)

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概要
カタログ番号HT381014
説明
Anti-MGAM/MGA Polyclonal Antibody (HT381014) is a rabbit polyclonal antibody detecting MGAM in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
種反応性Human
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant Human MGAM/MGA (Gly368-His731).
ターゲット Maltase-glucoamylase, intestinal, Glucan 1, 4-alpha-glucosidase, MGA, Alpha-glucosidase, MGAML, MGAM
エンドトキシンレベル Please contact with the lab for this information.
精製 Purified by antigen affinity column.
アクセッション番号 O43451
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Maltase-glucoamylase (MGAM/MGA) is a ~312 kDa protein. Alpha-(1,4) exo-glucosidase involved in breakdown of dietary starch oligosaccharides in small intestine. Cleaves the non-reducing alpha-(1,4)-linked glucose residue in linear dextrins with retention of anomeric center stereochemistry. Mainly hydrolyzes short length oligomaltoses having two to seven glucose residues. Can cleave alpha-(1,2), alpha-(1,3) and alpha-(1,6) glycosidic linkages with lower efficiency, whereas beta glycosidic linkages are usually not hydrolyzed.

1. Nichols, BL. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 1432-7. PMID: 12547908
2. Sim, L. et al. (2008) Journal of molecular biology 375, 782-92. PMID: 18036614
3. Quezada-Calvillo, R. et al. (2008) The Journal of nutrition 138, 685-92. PMID: 18356321
4. Ren, L. et al. (2011) Protein & cell 2, 827-36. PMID: 22058037
5. Lee, BH. et al. (2016) Journal of agricultural and food chemistry 64, 6487-94. PMID: 27480812
注意事項 For research use only.
イメージ
参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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