Anti-MLLT3 Polyclonal Antibody (HA633014)

Name: Anti-MLLT3 Polyclonal Antibody
Brand: abinScience
SKU: HA633014
Price: 96 USD
Availability: InStock

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Número de catálogo

HA633014

Descripción

Anti-MLLT3 Polyclonal Antibody (HA633014) is a rabbit polyclonal antibody detecting Protein AF-9; ALL1-fused gene from chromosome 9 protein; Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein; YEATS domain-containing protein 3; MLLT3; AF9; YEATS3 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant human MLLT3 (Met1-Thr148).

Objetivo

Protein AF-9; ALL1-fused gene from chromosome 9 protein; Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein; YEATS domain-containing protein 3; MLLT3; AF9; YEATS3

Nivel de endotoxinas

Please contact with the lab for this information.

Purificación

Purified by antigen affinity column.

Número de acceso

P42568, A2AM29

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Protein AF-9 (MLLT3) is a ~63 kDa protein. Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Specifically recognizes and binds acylated histone H3, with a preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr). Also recognizes and binds histone H3 acetylated and butyrylated at 'Lys-9' (H3K9ac and H3K9bu, respectively), but with lower affinity than crotonylated histone H3.

1. Lin, C. et al. (2010) Molecular cell 37, 429-37. PMID: 20159561

2. He, N. et al. (2010) Molecular cell 38, 428-38. PMID: 20471948

3. Li, Y. et al. (2014) Cell 159, 558-71. PMID: 25417107

4. Li, Y. et al. (2016) Molecular cell 62, 181-193. PMID: 27105114

5. Zhang, Q. et al. (2016) Structure (London, England : 1993) 24, 1606-12. PMID: 27545619

6. Li, X. et al. (2018) Nature chemical biology 14, 1140-1149. PMID: 30374167

7. Klein, BJ. et al. (2018) Nature communications 9, 4574. PMID: 30385749

9. Becht, DC. et al. (2025) Nature structural & molecular biology 32, 709-718. PMID: 39794553

Nota

For research use only

Imágenes

Western blot

Western blot analysis was performed using anti-AF9 polyclonal antibody at 1ug/mL on various samples.
Lane 1: THP-1 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Hela cell lysate

Referencias

Datasheet

Anti-MLLT3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-MLLT3 Polyclonal Antibody (HA633014)

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Imágenes

Referencias

Datasheet

SDS