Anti-MLLT3 Polyclonal Antibody (HA633014)

Name: Anti-MLLT3 Polyclonal Antibody
Brand: abinScience
SKU: HA633014
Price: 96 USD
Availability: InStock

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Numéro de catalogue

HA633014

Description

Anti-MLLT3 Polyclonal Antibody (HA633014) is a rabbit polyclonal antibody detecting Protein AF-9; ALL1-fused gene from chromosome 9 protein; Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein; YEATS domain-containing protein 3; MLLT3; AF9; YEATS3 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant human MLLT3 (Met1-Thr148).

Cible

Protein AF-9; ALL1-fused gene from chromosome 9 protein; Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein; YEATS domain-containing protein 3; MLLT3; AF9; YEATS3

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

P42568, A2AM29

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Protein AF-9 (MLLT3) is a ~63 kDa protein. Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Specifically recognizes and binds acylated histone H3, with a preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr). Also recognizes and binds histone H3 acetylated and butyrylated at 'Lys-9' (H3K9ac and H3K9bu, respectively), but with lower affinity than crotonylated histone H3.

1. Lin, C. et al. (2010) Molecular cell 37, 429-37. PMID: 20159561

2. He, N. et al. (2010) Molecular cell 38, 428-38. PMID: 20471948

3. Li, Y. et al. (2014) Cell 159, 558-71. PMID: 25417107

4. Li, Y. et al. (2016) Molecular cell 62, 181-193. PMID: 27105114

5. Zhang, Q. et al. (2016) Structure (London, England : 1993) 24, 1606-12. PMID: 27545619

6. Li, X. et al. (2018) Nature chemical biology 14, 1140-1149. PMID: 30374167

7. Klein, BJ. et al. (2018) Nature communications 9, 4574. PMID: 30385749

9. Becht, DC. et al. (2025) Nature structural & molecular biology 32, 709-718. PMID: 39794553

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-AF9 polyclonal antibody at 1ug/mL on various samples.
Lane 1: THP-1 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Hela cell lysate

Reférences

Fiche technique

Anti-MLLT3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-MLLT3 Polyclonal Antibody (HA633014)

Aperçu

Images

Reférences

Fiche technique

SDS