Anti-MLST8 Polyclonal Antibody (HV195014)

Name: Anti-MLST8 Polyclonal Antibody
Brand: abinScience
SKU: HV195014
Price: 96 USD
Availability: InStock

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Imágenes
Referencias
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SDS

Vista general

Número de catálogo

HV195014

Descripción

Anti-MLST8 Polyclonal Antibody (HV195014) is a rabbit polyclonal antibody detecting mLST8 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Danio rerio, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human MLST8 (Tyr68-Trp247).

Objetivo

GBL, TORC subunit LST8, Mammalian lethal with SEC13 protein 8, G protein beta subunit-like, Gable, Protein GbetaL, mLST8, LST8, MLST8, Target of rapamycin complex subunit LST8

Purificación

Purified by antigen affinity column.

Número de acceso

Q9BVC4

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Target of rapamycin complex subunit LST8 (MLST8) is a ~35 kDa protein. Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. In response to nutrients, mTORC1 is recruited to the lysosome membrane and promotes protein, lipid and nucleotide synthesis by phosphorylating several substrates, such as ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1). In the same time, it inhibits catabolic pathways by phosphorylating the autophagy initiation components ULK1 and ATG13, as well as transcription factor TFEB, a master regulators of lysosomal biogenesis and autophagy. The mTORC1 complex is inhibited in response to starvation and amino acid depletion.

1. Kim, DH. et al. (2003) Molecular cell 11, 895-904. PMID: 12718876

2. Sarbassov, DD. et al. (2004) Current biology : CB 14, 1296-302. PMID: 15268862

3. Jacinto, E. et al. (2004) Nature cell biology 6, 1122-8. PMID: 15467718

4. Coffman, K. et al. (2014) The Journal of biological chemistry 289, 4723-34. PMID: 24403073

5. Wang, B. et al. (2017) Nature 545, 365-369. PMID: 28489822

6. Yu, Z. et al. (2022) The Journal of biological chemistry 298, 102288. PMID: 35926713

Nota

For research use only.

Imágenes

Immunofluorescence

HepG2 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting MLST8 (HV195014) at a concentration of 10.8 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of MLST8 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

Referencias

Datasheet

Anti-MLST8 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

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