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| カタログ番号 | HV195014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-MLST8 Polyclonal Antibody (HV195014) is a rabbit polyclonal antibody detecting mLST8 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Danio rerio, and Rat.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human MLST8 (Tyr68-Trp247). | ||||||||
| ターゲット | GBL, TORC subunit LST8, Mammalian lethal with SEC13 protein 8, G protein beta subunit-like, Gable, Protein GbetaL, mLST8, LST8, MLST8, Target of rapamycin complex subunit LST8 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q9BVC4 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Target of rapamycin complex subunit LST8 (MLST8) is a ~35 kDa protein. Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. In response to nutrients, mTORC1 is recruited to the lysosome membrane and promotes protein, lipid and nucleotide synthesis by phosphorylating several substrates, such as ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1). In the same time, it inhibits catabolic pathways by phosphorylating the autophagy initiation components ULK1 and ATG13, as well as transcription factor TFEB, a master regulators of lysosomal biogenesis and autophagy. The mTORC1 complex is inhibited in response to starvation and amino acid depletion. 1. Kim, DH. et al. (2003) Molecular cell 11, 895-904. PMID: 12718876 2. Sarbassov, DD. et al. (2004) Current biology : CB 14, 1296-302. PMID: 15268862 3. Jacinto, E. et al. (2004) Nature cell biology 6, 1122-8. PMID: 15467718 4. Coffman, K. et al. (2014) The Journal of biological chemistry 289, 4723-34. PMID: 24403073 5. Wang, B. et al. (2017) Nature 545, 365-369. PMID: 28489822 6. Yu, Z. et al. (2022) The Journal of biological chemistry 298, 102288. PMID: 35926713 | ||||||||
| 注意事項 | For research use only. |
HepG2 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting MLST8 (HV195014) at a concentration of 10.8 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of MLST8 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
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