Anti-MLST8 Polyclonal Antibody (HV195014)

Name: Anti-MLST8 Polyclonal Antibody
Brand: abinScience
SKU: HV195014
Price: 96 USD
Availability: InStock

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Обзор

Каталожный номер

HV195014

Описание

Anti-MLST8 Polyclonal Antibody (HV195014) is a rabbit polyclonal antibody detecting mLST8 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Danio rerio, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human MLST8 (Tyr68-Trp247).

Цель

GBL, TORC subunit LST8, Mammalian lethal with SEC13 protein 8, G protein beta subunit-like, Gable, Protein GbetaL, mLST8, LST8, MLST8, Target of rapamycin complex subunit LST8

Очистка

Purified by antigen affinity column.

Номер доступа

Q9BVC4

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Target of rapamycin complex subunit LST8 (MLST8) is a ~35 kDa protein. Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. In response to nutrients, mTORC1 is recruited to the lysosome membrane and promotes protein, lipid and nucleotide synthesis by phosphorylating several substrates, such as ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1). In the same time, it inhibits catabolic pathways by phosphorylating the autophagy initiation components ULK1 and ATG13, as well as transcription factor TFEB, a master regulators of lysosomal biogenesis and autophagy. The mTORC1 complex is inhibited in response to starvation and amino acid depletion.

1. Kim, DH. et al. (2003) Molecular cell 11, 895-904. PMID: 12718876

2. Sarbassov, DD. et al. (2004) Current biology : CB 14, 1296-302. PMID: 15268862

3. Jacinto, E. et al. (2004) Nature cell biology 6, 1122-8. PMID: 15467718

4. Coffman, K. et al. (2014) The Journal of biological chemistry 289, 4723-34. PMID: 24403073

5. Wang, B. et al. (2017) Nature 545, 365-369. PMID: 28489822

6. Yu, Z. et al. (2022) The Journal of biological chemistry 298, 102288. PMID: 35926713

Примечание

For research use only.

Изображения

Immunofluorescence

HepG2 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting MLST8 (HV195014) at a concentration of 10.8 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of MLST8 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

Ссылки

Даташит

Anti-MLST8 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

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Formula

C₁ × V₁ = C₂ × V₂

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C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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