Anti-Mouse ASAH2 Polyclonal Antibody (MP220014)

Name: Anti-Mouse ASAH2 Polyclonal Antibody
Brand: abinScience
SKU: MP220014
Price: 168 USD
Availability: InStock

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Spécification :

50ug
100ug

Nombre :

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SDS

Aperçu

Numéro de catalogue

MP220014

Description

Anti-Mouse ASAH2 Polyclonal Antibody (MP220014) is a rabbit polyclonal antibody detecting ASAH2 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Mouse ASAH2 (Glu641-Thr756).

Cible

Neutral ceramidase (N-CDase) (NCDase) (EC 3.5.1.-) (EC 3.5.1.23) (Acylsphingosine deacylase 2) (N-acylsphingosine amidohydrolase 2) [Cleaved into: Neutral ceramidase soluble form]

Purification

Purified by antigen affinity column.

Numéro d'accès

Q9JHE3

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Neutral ceramidase (ASAH2) is a ~83 kDa protein. Plasma membrane ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at neutral pH. Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation. Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine. Together with sphingomyelinase, participates in the production of sphingosine and sphingosine-1-phosphate from the degradation of sphingomyelin, a sphingolipid enriched in the plasma membrane of cells. Also participates in the hydrolysis of ceramides from the extracellular milieu allowing the production of sphingosine-1-phosphate inside and outside cells.

1. Tani, M. et al. (2000) The Journal of biological chemistry 275, 3462-8. PMID: 10652340

2. Tani, M. et al. (2000) The Journal of biological chemistry 275, 11229-34. PMID: 10753931

3. Kono, M. et al. (2006) The Journal of biological chemistry 281, 7324-31. PMID: 16380386

4. Choi, MS. et al. (2003) Gene 315, 113-22. PMID: 14557071

5. Novgorodov, SA. et al. (2011) The Journal of biological chemistry 286, 25352-62. PMID: 21613224

6. Tani, M. et al. (2005) The Journal of biological chemistry 280, 36592-600. PMID: 16126722

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Mouse ASAH2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-Mouse ASAH2 Polyclonal Antibody (MP220014)

Aperçu

Images

Reférences

Fiche technique

SDS