Anti-Mouse CASP8 Polyclonal Antibody (MC266014)

Name: Anti-Mouse CASP8 Polyclonal Antibody
Brand: abinScience
SKU: MC266014
Price: 96 USD
Availability: InStock

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Datasheet
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Vista general

Número de catálogo

MC266014

Descripción

Anti-Mouse CASP8 Polyclonal Antibody (MC266014) is a rabbit polyclonal antibody detecting Casp8 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Mouse CASP8 (Gly236-Pro480).

Objetivo

Caspase-8, CASP-8, 3.4.22.61, Caspase-8 subunit p18, Caspase-8 subunit p10, Casp8

Purificación

Purified by antigen affinity column.

Número de acceso

O89110

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Caspase-8 (CASP8) is a ~55 kDa protein. Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood. Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for FAS/CD95-mediated and TNFRSF1A-induced cell death. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. Binding to the adapter molecule FADD recruits it to either receptor FAS/CD95 or TNFRSF1A. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation.

1. Benchoua, A. et al. (2002) The Journal of biological chemistry 277, 34217-22. PMID: 12065591

2. Sun, H. et al. (2008) Cell 133, 415-26. PMID: 18455983

3. Orning, P. et al. (2018) Science (New York, N.Y.) 362, 1064-1069. PMID: 30361383

4. Sarhan, J. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E10888-E10897. PMID: 30381458

5. Newton, K. et al. (2019) Nature 574, 428-431. PMID: 31511692

6. Fritsch, M. et al. (2019) Nature 575, 683-687. PMID: 31748744

7. Muendlein, HI. et al. (2021) Nature communications 12, 86. PMID: 33397971

8. Luo, X. et al. (1998) Cell 94, 481-90. PMID: 9727491

9. Li, H. et al. (1998) Cell 94, 491-501. PMID: 9727492

10. Rickard, JA. et al. (2014) Cell 157, 1175-88. PMID: 24813849

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-CASP8 polyclonal antibody at CASP8 on various samples.
Lane 1: Jurkat cell lysate
Lane 2: 293T cell lysate
Lane 3: HCT116 cell lysate
Lane 4: THP-1 cell lysate

Referencias

Datasheet

Anti-Mouse CASP8 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

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