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| カタログ番号 | MC266014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Mouse CASP8 Polyclonal Antibody (MC266014) is a rabbit polyclonal antibody detecting Casp8 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
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| 種反応性 | Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Mouse CASP8 (Gly236-Pro480). | ||||||||
| ターゲット | Caspase-8, CASP-8, 3.4.22.61, Caspase-8 subunit p18, Caspase-8 subunit p10, Casp8 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | O89110 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Caspase-8 (CASP8) is a ~55 kDa protein. Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood. Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for FAS/CD95-mediated and TNFRSF1A-induced cell death. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. Binding to the adapter molecule FADD recruits it to either receptor FAS/CD95 or TNFRSF1A. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. 1. Benchoua, A. et al. (2002) The Journal of biological chemistry 277, 34217-22. PMID: 12065591 2. Sun, H. et al. (2008) Cell 133, 415-26. PMID: 18455983 3. Orning, P. et al. (2018) Science (New York, N.Y.) 362, 1064-1069. PMID: 30361383 4. Sarhan, J. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E10888-E10897. PMID: 30381458 5. Newton, K. et al. (2019) Nature 574, 428-431. PMID: 31511692 6. Fritsch, M. et al. (2019) Nature 575, 683-687. PMID: 31748744 7. Muendlein, HI. et al. (2021) Nature communications 12, 86. PMID: 33397971 8. Luo, X. et al. (1998) Cell 94, 481-90. PMID: 9727491 9. Li, H. et al. (1998) Cell 94, 491-501. PMID: 9727492 10. Rickard, JA. et al. (2014) Cell 157, 1175-88. PMID: 24813849 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-CASP8 polyclonal antibody at CASP8 on various samples.
Lane 1: Jurkat cell lysate
Lane 2: 293T cell lysate
Lane 3: HCT116 cell lysate
Lane 4: THP-1 cell lysate
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